Confocal Raman microscopy in life sciences

Confocal Raman Microscopy in Pharmaceutical Development

Multiphoton microscopy in life sciences.

Near infrared (NIR) multiphoton microscopy is becoming a novel optical tool of choice for fluorescence imaging with high spatial and temporal resolution, diagnostics, photochemistry and nanoprocessing within living cells and tissues. Three-dimensional fluorescence imaging based on non-resonant two-photon or three-photon fluorophor excitation requires light intensities in the range of MW cm(-2) ...

Polarized Raman confocal microscopy of single gallium nitride nanowires.

Polarized Raman spectra and corresponding Raman scattering intensity images of an isolated gallium nitride nanowire with a diameter of 170 nm are presented. The sensitivity of the confocal microscope combined with a high-resolution piezoelectric stage enables analysis of the crystalline phase and crystallographic orientation of an individual nanowire with an excellent spatial and spectral resol...

Characterization of Developing Cotton Fibers by Confocal Raman Microscopy

Cellulose deposition in developing cotton fibers has been studied previously with analytical techniques, such as Fourier transform infrared spectroscopy (FTIR), High-performance liquid chromatography (HPLC) and Thermogravimetric analysis (TGA). Recent technological developments in instrumentation have made Raman microscopy emerge as an extraordinary analytical tool in biological and plant resea...

Composition of bovine milk fat globules by confocal Raman microscopy

Confocal Raman microscopy was used to determine the chemical fingerprint of individual bovine milk fat globules of different sizes and from milk sourced from different breeds. No detectable level of triglycerides was found in the small milk fat globules of 1 mm diameter or less in size. These small fat globules also delivered information about the lipids of the milk fat globule membrane. In add...